منابع مشابه
ELISA for thyroglobulin in serum: recovery studies to evaluate autoantibody interference and reliability of thyroglobulin values.
An ELISA for measuring thyroglobulin (Tg) in serum was developed with polyclonal antibodies to Tg on the solid phase and two monoclonal antibodies to Tg as the second antibodies. The assay has a detection limit of 1 microgram/L, a within-run imprecision (CV) of <7%, and a between-run CV of < 10%. Parallelism of the assay was shown in dilution studies, in which the percent observed/expected valu...
متن کاملA radioimmunoassay for serum rat thyroglobulin. Physiologic and pharmacological studies.
A double antibody radioimmunoassay has been developed to measure thyroglobulin in rat (RTg) serum. The lowest detectable quantity measurable was 5.0 ng/ml. Specificity was documented by: (a) fall in serum RTg to undetectable levels after thyroid ablation; (b) the fact that L-thyroxine, D-thyroxine, L-triiodothyronine, D-triiodothyronine, triiodothyroacetic acid, tetraiodothyroacetic acid, triio...
متن کاملThe Properties of Thyroglobulin VIII. THE IODINATION OF THYROGLOBULIN
It has been shown that when thyroglobulin is iodinated, a marked intensification occurs in its absorption spectrum in the region of 300 to 3fXl rnp in alkaline solution (1). It is also well known that the absorption peaks of iodinated tyrosine and thyronine derivatives occur in this spectral zone (2). A procedure based on these factors has been developed which permits the simultaneous determina...
متن کاملSubunit heterogeneity of thyroglobulin.
The subunit structure of thyroglobulin has been investigated by gel filtration and polyacrylamide electrophoresis in sodium dodecyl sulfate (SDS) of the native and the S-carboxymethylated proteins. When the thyroglobulins from several species (calf, sheep, pig, man, rat, and guinea pig) were examined by polyacrylamide electrophoresis in SDS in the absence of reducing agents, most of the protein...
متن کاملExposure of thyroxine residues in human thyroglobulin. Two-site binding studies.
Human thyroglobulin (Tg) could be adsorbed through one of its thyroxine (T4) residues by either of two T4-binding antibodies which had been covalently attached to Sepharose- CL4B . The antibodies used were (i) a purified human autoantibody specific for a T4-containing epitope in human Tg, or (ii) a rabbit antibody raised against T4 conjugated to bovine albumin side chains. Tg adsorbed by either...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1936
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)65197-3